Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212904_v18h TGTTAGGAGTAGGTTCCTGGGCCTGGAAACAG(+CAGTGT)GATCCTGGTTCAACCCTTCCAGCAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605212902_9d8i GCTTCTCCATCTTGCTGGAAGGGTTGAACCAG(+CAGTGT)GATCCTGTTTCCAGGCCCAGGAACCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240605212900_9iee CCTTGATGCAGTCTGTGCCTGACCGCTGCCTG(+CAGTGT)GTACCAACAGTAACACTACAAAAGGCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605212859_t056 AGTCCAGAGAGCCTTTTGTAGTGTTACTGTTG(+CAGTGT)GTACCAGGCAGCGGTCAGGCACAGACTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605212857_544h AGGGTAAATAGGCAACATTGCCCCTAAGCCTG(+CAGTGT)GATCCTAAAGATGGTTCTAGCCATGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605212854_jsqj TACAGGAAGGGCATGGCTAGAACCATCTTTAG(+CAGTGT)GATCCAGGCTTAGGGGCAATGTTGCCTATTT NGG 10 8 17 8 24 0 100 5 10
    240605212853_m25b GTTTTCCTAAAGAACTTCTCACATCTCAAGAG(+CAGTGT)GATCCCGGTTGAGCATCATTTGTGTAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605212851_rh2h CATCACCACCTACACAAATGATGCTCAACCGG(+CAGTGT)GATCCTCTTGAGATGTGAGAAGTTCTTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605212848_1lqs GGTCTGCAATATTTCTTAAGAATTTACCCTTG(+CAGTGT)GATCCCAATAAGCATGGAGACATGTCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605212847_ahin AGACATTGGAGACATGTCTCCATGCTTATTGG(+CAGTGT)GATCCAAGGGTAAATTCTTAAGAAATATTGC NGG 10 8 17 8 24 0 100 5 10