Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212828_ode6 TGGGGCCCAGCTGCGGCTGGCTCCCGGCCTGG(+CAGTGT)GTACCAGAAAGAGACCTTCAGCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605212827_zmg8 CCAGCCTGGGTTGAGCTGAAGGTCTCTTTCTG(+CAGTGT)GTACCCAGGCCGGGAGCCAGCCGCAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605212825_bb4r TCTGTTGTTACTAATAACAGACTTTATCATGG(+CAGTGT)GTACCAGCCATAGCGAGTTAATAAGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240605212823_7ow1 TGGATATGTCACTTATTAACTCGCTATGGCTG(+CAGTGT)GTACCCATGATAAAGTCTGTTATTAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240605212821_rb2m TTCGACATTTTAACCTGTTCTGCCTGACATGG(+CAGTGT)GATCCTCCATCCCAGCTCCTTCCCCAACACT NGG 10 8 17 8 24 0 100 5 10
    240605212820_1p51 AGCTCAGTGTTGGGGAAGGAGCTGGGATGGAG(+CAGTGT)GATCCCATGTCAGGCAGAACAGGTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605212817_q2d0 TTGATGCCTTCTTTTAAAGTACCCTGCTCCTG(+CAGTGT)GTACCCAGTTAATCCCCTCTCCCAGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240605212815_4qdy CCGACTTTATACTGGGAGAGGGGATTAACTGG(+CAGTGT)GTACCAGGAGCAGGGTACTTTAAAAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605212814_al2f GTGCAACCTGTTACCTTACACAGTGCCTCATG(+CAGTGT)GTACCCTGGGAGTGTGCGGGCATCATTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605212810_y5vc ACGGTTGGGAATGATGCCCGCACACTCCCAGG(+CAGTGT)GTACCATGAGGCACTGTGTAAGGTAACAGGT NGG 10 8 17 8 24 0 100 5 10