Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212718_ci99 AAGAAGGAGGAGGAGGGATGAGTGGTCTAGTG(+CAGTGT)GTACCCTCGTGCCAACAGGGTTCGTGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605212716_vj8m TTTGCACCACCACGAACCCTGTTGGCACGAGG(+CAGTGT)GTACCACTAGACCACTCATCCCTCCTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605212715_1mln TCATAAAAAATAACTCATAGGTACAAGTAATG(+CAGTGT)GTACCATATGAGTATGAGTGTAGCCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605212713_km4t GCTCAGAGAAAGGCTACACTCATACTCATATG(+CAGTGT)GTACCATTACTTGTACCTATGAGTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605212712_zhas CAGAAAATGTTCTTAGGGCATCATGTCACAAG(+CAGTGT)GATCCTTGTAACATATTGGCTCAAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605212710_uepx AGTCTAGAAAATTTGAGCCAATATGTTACAAG(+CAGTGT)GATCCTTGTGACATGATGCCCTAAGAACATT NGG 10 8 17 8 24 0 100 5 10
    240605212708_p52y GGTGAGTACCACTATGCTTGGCTTCCCTTTTG(+CAGTGT)GATCCTCTCCCCCTTTCAGCAAAAAAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605212707_tt0d AGGGTAACTTTTTTTTGCTGAAAGGGGGAGAG(+CAGTGT)GATCCAAAAGGGAAGCCAAGCATAGTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605212705_27y4 GCTGCATGAATGAAATGATGTGATGATGGAGG(+CAGTGT)GATCCTGCTTTCTAAACTTTATGAACTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605212704_1t91 TGGCTTGAGAGTTCATAAAGTTTAGAAAGCAG(+CAGTGT)GATCCCTCCATCATCACATCATTTCATTCAT NGG 10 8 17 8 24 0 100 5 10