Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212809_adp5 GTGAGTTTACCATTTCACATTATCATCACTAG(+CAGTGT)GATCCTCTCCTTTCCAGATGGAAGCTCACAC NGG 10 8 17 8 24 0 100 5 10
    240605212807_ygnb CATGGGTGTGAGCTTCCATCTGGAAAGGAGAG(+CAGTGT)GATCCTAGTGATGATAATGTGAAATGGTAAA NGG 10 8 17 8 24 0 100 5 10
    240605212805_i916 AAAATTACATGTCCATGAACAGACTTTTCCTG(+CAGTGT)GATCCAGACCACAAAAAGGGAATTATTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605212803_h5mw TATGTGAAAAATAATTCCCTTTTTGTGGTCTG(+CAGTGT)GATCCAGGAAAAGTCTGTTCATGGACATGTA NGG 10 8 17 8 24 0 100 5 10
    240605212802_7jwj GAATTTAAGAAGGTAATGAAAGCTAAGCTAAG(+CAGTGT)GTACCTGTGACAAGTGTTTGGTAATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605212801_69e8 CTTAGTATTTTATTACCAAACACTTGTCACAG(+CAGTGT)GTACCTTAGCTTAGCTTTCATTACCTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605212800_jwaq ATTGCTTCACATGACAGCTGTTCTGACTGCAG(+CAGTGT)GTACCTTCACTGCCTTTGCTGCACAGTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605212758_amtm CTGATCCTCACTGTGCAGCAAAGGCAGTGAAG(+CAGTGT)GTACCTGCAGTCAGAACAGCTGTCATGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605212755_9sro GATCACTAATGACAGGTCGCTTTCCTCCAGGG(+CAGTGT)GTACCACTGAGCTACAGCAGGACATCATGGC NGG 10 8 17 8 24 0 100 5 10
    240605212754_38e0 GCTCGGCCATGATGTCCTGCTGTAGCTCAGTG(+CAGTGT)GTACCCCTGGAGGAAAGCGACCTGTCATTAG NGG 10 8 17 8 24 0 100 5 10