Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212736_4vpk AGACTCATTAATAGGATTGTTAGCAACCACTG(+CAGTGT)GATCCTCTGGTGGATAGACAAATGTTCTATA NGG 10 8 17 8 24 0 100 5 10
    240605212734_pitb TTCCATATAGAACATTTGTCTATCCACCAGAG(+CAGTGT)GATCCAGTGGTTGCTAACAATCCTATTAATG NGG 10 8 17 8 24 0 100 5 10
    240605212732_6yn4 ATGAGATGAAATATTTGCTTCGCACACGCAGG(+CAGTGT)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605212730_voc9 AAGGTCTCCTTTGAAGAGAACTTCTATAGTGG(+CAGTGT)GATCCCTGCGTGTGCGAAGCAAATATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605212728_xkuo AAGTGAAGGCTATGATTATTTCTCTAGTGCAG(+CAGTGT)GATCCGGACAGGGTTCCACAAATCAATTCCT NGG 10 8 17 8 24 0 100 5 10
    240605212726_fvkv GATATAGGAATTGATTTGTGGAACCCTGTCCG(+CAGTGT)GATCCTGCACTAGAGAAATAATCATAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605212725_n8n7 ATCCCAGAAAAATAGGAAGTCATTATAAATAG(+CAGTGT)GATCCAATGGAACAATGTTCTTCAAATAATA NGG 10 8 17 8 24 0 100 5 10
    240605212723_veuj AGTTATATTATTTGAAGAACATTGTTCCATTG(+CAGTGT)GATCCTATTTATAATGACTTCCTATTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605212721_1ajv AAACAGAGGATGGCTTCAAACAGGCAAGTTTG(+CAGTGT)GTACCTCTTAGTGACAATTCACGGAGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605212720_r5d1 AATTTCCTTTCTCCGTGAATTGTCACTAAGAG(+CAGTGT)GTACCAAACTTGCCTGTTTGAAGCCATCCTC NGG 10 8 17 8 24 0 100 5 10