Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212645_881b GCAATCACATCGTGGCCAACCATCTGTAATGG(+CAGTGT)GATCCAATGCCCTCTTCAGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605212644_m2i6 GCTGTCTTCAGACATACCTGAAGAGGGCATTG(+CAGTGT)GATCCCATTACAGATGGTTGGCCACGATGTG NGG 10 8 17 8 24 0 100 5 10
    240605212642_fr1w TAGCATTCAAGCCATTCTTAGGAAGGACCAGG(+CAGTGT)GATCCTCTCTCATCTCTTGGGGTTGCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605212640_s814 CCTAGGAAAGGCAACCCCAAGAGATGAGAGAG(+CAGTGT)GATCCCTGGTCCTTCCTAAGAATGGCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605212639_c9oj GCAGTAGATAATCAGGAAGATACAATGACTTG(+CAGTGT)GATCCTAGAACCACCTTCCTTTTCCTTTATG NGG 10 8 17 8 24 0 100 5 10
    240605212637_v8pi TCTTTCATAAAGGAAAAGGAAGGTGGTTCTAG(+CAGTGT)GATCCAAGTCATTGTATCTTCCTGATTATCT NGG 10 8 17 8 24 0 100 5 10
    240605212636_06pw TGTAGAAACTGGTGGTGTAGAAAGATACTGAG(+CAGTGT)GATCCAAGTTGTGTACTAGGACATGATTGAC NGG 10 8 17 8 24 0 100 5 10
    240605212635_koo0 CTTGTGTCAATCATGTCCTAGTACACAACTTG(+CAGTGT)GATCCTCAGTATCTTTCTACACCACCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605212633_wa7j TGTCACAGTCTGGTCTTTCTAGGAAAATTCTG(+CAGTGT)GATCCAGTAAGAGTTAAGCATTGAAAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605212632_082i AATCAGCTCTTTTCAATGCTTAACTCTTACTG(+CAGTGT)GATCCAGAATTTTCCTAGAAAGACCAGACTG NGG 10 8 17 8 24 0 100 5 10