Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212557_b7fa CTCTCACCTGGGAACTGAGCTCACACACAGGG(+CAGTGT)GTACCGGATGGCCACACTTCCTGCTCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605212555_tqdu CCACGGTGAGGAGCAGGAAGTGTGGCCATCCG(+CAGTGT)GTACCCCTGTGTGTGAGCTCAGTTCCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605212552_qjpw TTCTGATCTAGATCTCTGGCTGGACATTTGGG(+CAGTGT)GATCCCGTTTGGAGGTGATGGATCTCACTGT NGG 10 8 17 8 24 0 100 5 10
    240605212551_y8mv CATATACAGTGAGATCCATCACCTCCAAACGG(+CAGTGT)GATCCCCAAATGTCCAGCCAGAGATCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605212550_7d5b GGACTCCGGTCCAATCTGGAGCATGGGTAGAG(+CAGTGT)GATCCAGAGACGACAAAGCTAGAGAAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605212548_zpc0 TGGCTCTGCCTTCTCTAGCTTTGTCGTCTCTG(+CAGTGT)GATCCTCTACCCATGCTCCAGATTGGACCGG NGG 10 8 17 8 24 0 100 5 10
    240605212544_rxbe TTTGGCATGCAAGTACAAGGACCTCAGTTTGG(+CAGTGT)GATCCCCATTGCCAATCTAAAAAGCTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605212543_3tgo CACTGCATCTAGCTTTTTAGATTGGCAATGGG(+CAGTGT)GATCCCAAACTGAGGTCCTTGTACTTGCATG NGG 10 8 17 8 24 0 100 5 10
    240605212541_y13h GGTCTCAGCGTTTGGAAGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605212538_d1oy GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10