Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212444_1i3t CCTGGGACATAAATGTCCCCACAGCCAGGTTG(+CAGTGT)GTACCATTCAGTGTTTATTTAGACACACATA NGG 10 8 17 8 24 0 100 5 10
    240605212443_c9u5 ACAACCTACCTGCTCTGCTGTTTCTGGAGTTG(+CAGTGT)GTACCATACAGTTCCCTGGCATGGAAGGATC NGG 10 8 17 8 24 0 100 5 10
    240605212441_v1qa GGCCCGATCCTTCCATGCCAGGGAACTGTATG(+CAGTGT)GTACCAACTCCAGAAACAGCAGAGCAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605212440_yfl6 CATTTAGGGTACACTGGCAAAGGAAGGGGCTG(+CAGTGT)GATCCTAGTTTGGCTAAAAGGCAGGCTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605212439_yvmp ACTCATTGCAGCCTGCCTTTTAGCCAAACTAG(+CAGTGT)GATCCAGCCCCTTCCTTTGCCAGTGTACCCT NGG 10 8 17 8 24 0 100 5 10
    240605212437_xhbj AGTGTCAGGTCTGACTGCAGACGCTCTTTAGG(+CAGTGT)GTACCCACTCTTCACCCTCACATAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240605212435_j5vo TGTGTGTAGTGTTATGTGAGGGTGAAGAGTGG(+CAGTGT)GTACCCTAAAGAGCGTCTGCAGTCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240605212434_1g8s GAACCTGGAATAGCTGTCGGGGCTTCAGCGAG(+CAGTGT)GATCCCTCCAAACCTCATGTTCTGTCCCGAT NGG 10 8 17 8 24 0 100 5 10
    240605212432_812g GCGACATCGGGACAGAACATGAGGTTTGGAGG(+CAGTGT)GATCCTCGCTGAAGCCCCGACAGCTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240605212430_rcrs GCTCTGCAGAATCCAGCTTAATCCAGAAACAG(+CAGTGT)GTACCTAAAAGAGATGGCGTCGCTCTTCTTT NGG 10 8 17 8 24 0 100 5 10