Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214246_5nho ATATGGCTATAAATATAAATTTTGTATTACTG(+CAGTGT)GTACCAAAAACACTTTTTTTTTTTTTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605214244_n0gv CCCTGCTGGAAAAAAAAAAAAAAGTGTTTTTG(+CAGTGT)GTACCAGTAATACAAAATTTATATTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240605214243_idiz CTAGGTGTTCAGGGACCTCCAAAAGCTCCAAG(+CAGTGT)GATCCACCTCTTGCTACATCTTCAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605214241_vlkf CTCTATTCCTACTGAAGATGTAGCAAGAGGTG(+CAGTGT)GATCCTTGGAGCTTTTGGAGGTCCCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240605214239_velh TTGAGCGAGCGAGAGATTATTCTAATGTCCTG(+CAGTGT)GTACCCGGCTGGCTTCTGGGAGGACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605214237_bb2r TCGAAAAGCCAGTCCTCCCAGAAGCCAGCCGG(+CAGTGT)GTACCAGGACATTAGAATAATCTCTCGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605214236_ljzy AAGCTTATTGGCCAAACCCTCAGGGCCTCTAG(+CAGTGT)GTACCTCATTAGCATGGAGAACTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605214234_zbgc ATCATCAGAACAGAGTTCTCCATGCTAATGAG(+CAGTGT)GTACCTAGAGGCCCTGAGGGTTTGGCCAATA NGG 10 8 17 8 24 0 100 5 10
    240605214233_ekco TATGTGGGTGTGAGGTGGTCAGGCCATTTGTG(+CAGTGT)GTACCCGTAGTGTTTGGTGCTGGCCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240605214231_xzeg ATAGTGGCTAGGGCCAGCACCAAACACTACGG(+CAGTGT)GTACCACAAATGGCCTGACCACCTCACACCC NGG 10 8 17 8 24 0 100 5 10