Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212413_g2op TTCCTGATCAAAGCCACTTTAATGAGAAATAG(+CAGTGT)GTACCAGTCCTCAGCAGGGGCCAGGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605212412_jajj TTCCACCTCTTATCAGGAGCTGCCCTGGAAAG(+CAGTGT)GATCCGGTCATGCTGCCTGCCTCTTTTCATC NGG 10 8 17 8 24 0 100 5 10
    240605212410_a90j TGACAGATGAAAAGAGGCAGGCAGCATGACCG(+CAGTGT)GATCCTTTCCAGGGCAGCTCCTGATAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605212407_jlw6 GAGTCAATAATGTGAGGGTGGTGGGGTGGGGG(+CAGTGT)GATCCTCAGTACAAGAAAACTGAATAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605212406_u127 TCCAAAACACTATTCAGTTTTCTTGTACTGAG(+CAGTGT)GATCCCCCCACCCCACCACCCTCACATTATT NGG 10 8 17 8 24 0 100 5 10
    240605212404_a39p AAGGGTCTCTCACTAAAACTGAAGCTACGTTG(+CAGTGT)GTACCCCAGAACGCCCCAGTCGTTCTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605212402_mh0f AACGGGCAAGAGAACGACTGGGGCGTTCTGGG(+CAGTGT)GTACCAACGTAGCTTCAGTTTTAGTGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605212401_0tx4 CTCAGTTGGCAGAGTGCTAGCCTAGATGCATG(+CAGTGT)GATCCTTGGGCTCAGGCCTCAGCACTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605212358_cjwl TGCCTAGTTCAGTGCTGAGGCCTGAGCCCAAG(+CAGTGT)GATCCATGCATCTAGGCTAGCACTCTGCCAA NGG 10 8 17 8 24 0 100 5 10
    240605212356_s64t CCACCCCACCACAGAATGCTGATATGCCAGTG(+CAGTGT)GTACCCAGTACCTCTTGTATATAGTTATTGC NGG 10 8 17 8 24 0 100 5 10