Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212354_gcc5 TATTTGCAATAACTATATACAAGAGGTACTGG(+CAGTGT)GTACCACTGGCATATCAGCATTCTGTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605212352_v9og ATGCTGTTCACATGGTGGGTATAACTTTCAAG(+CAGTGT)GTACCACCCTGGCCTGCACCTGCCGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605212350_5evg TGGGTCCTGACCGGCAGGTGCAGGCCAGGGTG(+CAGTGT)GTACCTTGAAAGTTATACCCACCATGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605212347_wute CTCTTCAAGCCCTCCTTTCAGTGGGGCCCTGG(+CAGTGT)GTACCATGATGGCCTCCCTGTCTTCTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605212346_ckb6 GACACTGCCTAGAAGACAGGGAGGCCATCATG(+CAGTGT)GTACCCAGGGCCCCACTGAAAGGAGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605212344_s116 ACCCTAAAGTTTGGAATGGGAACATGTGCATG(+CAGTGT)GATCCAACAACCAACCCAACCCTCAAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605212342_90bu GGACCGGAACTTGAGGGTTGGGTTGGTTGTTG(+CAGTGT)GATCCATGCACATGTTCCCATTCCAAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605212340_6qjz GGCCACCGACACTGGGTAGGGATAGCCACGGG(+CAGTGT)GTACCTGGTGTAATGTCCCTACCAGCTGACT NGG 10 8 17 8 24 0 100 5 10
    240605212338_x9m2 CTCTCAGTCAGCTGGTAGGGACATTACACCAG(+CAGTGT)GTACCCCGTGGCTATCCCTACCCAGTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240605212336_6yzf CATAACAACTGCAGGTGCTCAGACCACTGTTG(+CAGTGT)GTACCAGAACCAGTGCCTTGGTGGGGCCATC NGG 10 8 17 8 24 0 100 5 10