Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212429_ph1a AACAAAAAGAAGAGCGACGCCATCTCTTTTAG(+CAGTGT)GTACCTGTTTCTGGATTAAGCTGGATTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605212427_1vzs TATTCCGGTTGAGGAGTAGACAACTGACTGGG(+CAGTGT)GATCCAAACCAATTTTTACATGGCCTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605212426_m0nt TCACCCCTCAAGGCCATGTAAAAATTGGTTTG(+CAGTGT)GATCCCCAGTCAGTTGTCTACTCCTCAACCG NGG 10 8 17 8 24 0 100 5 10
    240605212424_3eik AGTCTTAGCTTGCTTCCTGGTATTCGTGGTAG(+CAGTGT)GATCCTGTCACAGGTCAATGTTAAGACTCAA NGG 10 8 17 8 24 0 100 5 10
    240605212423_2g09 AAATTTTGAGTCTTAACATTGACCTGTGACAG(+CAGTGT)GATCCTACCACGAATACCAGGAAGCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605212421_sacl ACAGGTCAACAACTTTCTTTCTTGGTTTCCTG(+CAGTGT)GTACCCTAGCCCCCCTCACACCCGTCTCACC NGG 10 8 17 8 24 0 100 5 10
    240605212419_co7u GCAAAGGTGAGACGGGTGTGAGGGGGGCTAGG(+CAGTGT)GTACCAGGAAACCAAGAAAGAAAGTTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605212417_clol CATCTGAGTTCATGCATCGAGCCTGGGCCTGG(+CAGTGT)GATCCAGTGAAGCTAAGCCCTCCTCCACCCA NGG 10 8 17 8 24 0 100 5 10
    240605212416_obcg GGCCCTGGGTGGAGGAGGGCTTAGCTTCACTG(+CAGTGT)GATCCCAGGCCCAGGCTCGATGCATGAACTC NGG 10 8 17 8 24 0 100 5 10
    240605212414_0t4e CCCTGAGGGATCCTGGCCCCTGCTGAGGACTG(+CAGTGT)GTACCTATTTCTCATTAAAGTGGCTTTGATC NGG 10 8 17 8 24 0 100 5 10