Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212631_yvyp TTCCCAGTTAGTTAGGGGTATGCTCCAAGAAG(+CAGTGT)GATCCTAGAGGTCCTGTGGGGATGTTGAATT NGG 10 8 17 8 24 0 100 5 10
    240605212629_ug7a ACTACAATTCAACATCCCCACAGGACCTCTAG(+CAGTGT)GATCCTTCTTGGAGCATACCCCTAACTAACT NGG 10 8 17 8 24 0 100 5 10
    240605212628_1hyd GAAGTGTGCCCAAGGTTAGAGAGTACAGAGTG(+CAGTGT)GATCCCCCTCCCCCCCAAAAAAGATAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240605212626_grca AGAACATTACTATCTTTTTTGGGGGGGAGGGG(+CAGTGT)GATCCACTCTGTACTCTCTAACCTTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605212624_3ra3 CTTTGTGGATACCTAGAAGGAGGTCTTGAGAG(+CAGTGT)GATCCAGTATTAATGGTGTCATAGAGACCAG NGG 10 8 17 8 24 0 100 5 10
    240605212623_r2iw GCTCTCTGGTCTCTATGACACCATTAATACTG(+CAGTGT)GATCCTCTCAAGACCTCCTTCTAGGTATCCA NGG 10 8 17 8 24 0 100 5 10
    240605212622_tnom TGGCCACAAGTAACAAGAGTGGCAAGAATCTG(+CAGTGT)GATCCCCAAGCTTCAGCCAACCAACAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605212620_hcz6 TCCATTAACCTGTTGGTTGGCTGAAGCTTGGG(+CAGTGT)GATCCAGATTCTTGCCACTCTTGTTACTTGT NGG 10 8 17 8 24 0 100 5 10
    240605212618_dec2 GGTAATTGAGTTTTGAATCTTATTAACTTATG(+CAGTGT)GATCCATCATACTTTCAAGTTTCCATAACAT NGG 10 8 17 8 24 0 100 5 10
    240605212617_uupt AATGTATGTTATGGAAACTTGAAAGTATGATG(+CAGTGT)GATCCATAAGTTAATAAGATTCAAAACTCAA NGG 10 8 17 8 24 0 100 5 10