Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212153_s0yc CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+CAGTGT)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212152_gwq7 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212150_juq1 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+CAGTGT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212148_ci1o TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+CAGTGT)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212147_b2wt CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+CAGTGT)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212146_m4af CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212144_8s4t TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+CAGTGT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212143_89rf CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212141_1xgr TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+CAGTGT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212139_nhij CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10