Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212137_j94u TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+CAGTGT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212136_b7ak CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+CAGTGT)GATCCAAGGTTTTTCAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212134_k5mc TGGAGATCAGTCAACAAAATTGAAAAACCTTG(+CAGTGT)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212133_pfip CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212131_fbrs TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+CAGTGT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212128_vm7w GGAATGACCATCCAGAGACTGCTTCACCTGGG(+CAGTGT)GATCCATCTCATAAACAACCAGAAAAACCAG NGG 10 8 17 8 24 0 100 5 10
    240605212127_z48c AGTGTCTGGTTTTTCTGGTTGTTTATGAGATG(+CAGTGT)GATCCCCAGGTGAAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605212125_t9wq CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605212124_fbx2 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+CAGTGT)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605212122_hilf CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+CAGTGT)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10