Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212049_hcs1 CCAAAATGAGTCGTTGCACTTAATTGCTAACG(+CAGTGT)GATCCTACCTTGTGTGAAACAATCAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240605212048_i8td AAAACACTAAGAATGCATTTTAGCCAGGTATG(+CAGTGT)GTACCAGGAAAGAGATGGCACATAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605212047_1ney TCTGGGTGCCTTTATGTGCCATCTCTTTCCTG(+CAGTGT)GTACCATACCTGGCTAAAATGCATTCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605212045_s5fa ATTCCTAAATTTAAAATGGGTGCTTGTCTTAG(+CAGTGT)GATCCTCCTATCAAGATACCAGTGAGGAACC NGG 10 8 17 8 24 0 100 5 10
    240605212043_e9th TCGGAGGTTCCTCACTGGTATCTTGATAGGAG(+CAGTGT)GATCCTAAGACAAGCACCCATTTTAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605212042_uxq4 TTAGATCACGGGTGACACTCGGTGACCTTGTG(+CAGTGT)GATCCCATAAGGTGGAAAGAGTATTTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605212041_e4j9 TCACACTGGAAAATACTCTTTCCACCTTATGG(+CAGTGT)GATCCACAAGGTCACCGAGTGTCACCCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605212038_ny8h ATTCTGCCATGTGGCTGATTACTTGTGCTCAG(+CAGTGT)GTACCATATGTCTGTTTCCTCACCTCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605212037_kxp6 CTCCCAGGAAGAGGTGAGGAAACAGACATATG(+CAGTGT)GTACCTGAGCACAAGTAATCAGCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240605212036_00mz AGAATGTGAGGCAGTGCTCAGGTACTGATGAG(+CAGTGT)GTACCTGAAGCAGTAAAGGAGTGTGGAGTGT NGG 10 8 17 8 24 0 100 5 10