Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211938_qtbp AGCGTTAGATTCTTAGCCCGTAGAGGACAGTG(+CAGTGT)GTACCAGGGTTTCAGAGCGGTCTCTGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605211937_wgpu TTAAAGAAACATTTGTCCAAATAGGCAAAGTG(+CAGTGT)GATCCCGACTTCAAACACATGAACTAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605211935_bh34 AATGTTCTACTAGTTCATGTGTTTGAAGTCGG(+CAGTGT)GATCCACTTTGCCTATTTGGACAAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605211933_lkba ACAATGCAGACAAATTTTTATAAGATGCTTGG(+CAGTGT)GATCCATAACAAGACTTTGTTCTATAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605211932_3tza TTTTTCCTCTTATAGAACAAAGTCTTGTTATG(+CAGTGT)GATCCCAAGCATCTTATAAAAATTTGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605211930_hbwf GAGTCCAACAGTGTTAAGACTCTTGAAATACG(+CAGTGT)GTACCTGGCAATGTTTGTTTCCTAATGAATT NGG 10 8 17 8 24 0 100 5 10
    240605211928_12xx TTCACAATTCATTAGGAAACAAACATTGCCAG(+CAGTGT)GTACCGTATTTCAAGAGTCTTAACACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605211925_8gz3 TGCACAACAGAGGCGATGAAGCGCTGCCCTCG(+CAGTGT)GATCCAGTTCCTCACCTTCAAGCCACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605211923_v554 GAGGCCTTGGGTGGCTTGAAGGTGAGGAACTG(+CAGTGT)GATCCGAGGGCAGCGCTTCATCGCCTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605211921_fke6 GGGGGGAAGTAAGGTGGTGTGCACTTTCTAGG(+CAGTGT)GATCCCAGAGAAGGGAGGAGCCAGGCTGTTT NGG 10 8 17 8 24 0 100 5 10