Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213720_erzj TGTCATTACGGAGGTGCTGAAAATCAAACACG(+CAGTGT)GATCCTCCGGAAGAGCAGCTAGTGCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605213717_wbm8 AGTGGTTAAAAGCACTAGCTGCTCTTCCGGAG(+CAGTGT)GATCCGTGTTTGATTTTCAGCACCTCCGTAA NGG 10 8 17 8 24 0 100 5 10
    240605213715_dowk ACACAAAGGCACAGCAAGACGGTGCTGCGTTG(+CAGTGT)GATCCTATTGCACTTCTCCATTCACTGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605213713_5fmh GGAGAGAACCAGTGAATGGAGAAGTGCAATAG(+CAGTGT)GATCCAACGCAGCACCGTCTTGCTGTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605213712_i032 GTTCTGTATATGCACACAGAGACCGCACTGTG(+CAGTGT)GTACCCTCGTTAAGGTGTTGCTCATGAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605213710_d3iy TTAGGGACTTCATGAGCAACACCTTAACGAGG(+CAGTGT)GTACCACAGTGCGGTCTCTGTGTGCATATAC NGG 10 8 17 8 24 0 100 5 10
    240605213709_4quc GTGCTGGAATGGGAAACTCAAGCTGGGAGGAG(+CAGTGT)GATCCCAAAGTACCGAAAAGGGGAAACTGAA NGG 10 8 17 8 24 0 100 5 10
    240605213707_0kn2 TTTCGTTCAGTTTCCCCTTTTCGGTACTTTGG(+CAGTGT)GATCCTCCTCCCAGCTTGAGTTTCCCATTCC NGG 10 8 17 8 24 0 100 5 10
    240605213706_1moc AGTAGGGTTTTACTTTTATAATTTTCTTCATG(+CAGTGT)GATCCATGAACAAATTATCTGACAGTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605213704_e6ba GGGGGGGGACACTGTCAGATAATTTGTTCATG(+CAGTGT)GATCCATGAAGAAAATTATAAAAGTAAAACC NGG 10 8 17 8 24 0 100 5 10