Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211846_06vi ATTTGTTTCCTTTTATTTCCAACTTTTTGGGG(+CAGTGT)GTACCTTAAATTGTAACTTCCAAGAACCTGC NGG 10 8 17 8 24 0 100 5 10
    240605211845_q24l ACATACACAGCTACAGAGTGAGAGTTGGTTTG(+CAGTGT)GATCCTCTTCATTTCATTTGTTTGCCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605211844_qk2d ATAACAGAAAGGCAAACAAATGAAATGAAGAG(+CAGTGT)GATCCAAACCAACTCTCACTCTGTAGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605211841_svbo CTCTTGTGTGTCCTGCCATCAGTGTGACCAGG(+CAGTGT)GTACCTCAATAAAGAGTGGTCGAGGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605211840_h6qh GAGAAACCTTTCCTCGACCACTCTTTATTGAG(+CAGTGT)GTACCCTGGTCACACTGATGGCAGGACACAC NGG 10 8 17 8 24 0 100 5 10
    240605211839_ilvq ACCTAACTGTGCAGGCTGACGATTAATTACTG(+CAGTGT)GATCCAAGAGAACAATACATTCTTATGTACA NGG 10 8 17 8 24 0 100 5 10
    240605211837_ir4h TCTTGTGTACATAAGAATGTATTGTTCTCTTG(+CAGTGT)GATCCAGTAATTAATCGTCAGCCTGCACAGT NGG 10 8 17 8 24 0 100 5 10
    240605211836_jkpg GGTTCTATATAGCCATATAATATTTTGATATG(+CAGTGT)GTACCTTGCAACACTGCATGTTCTTGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605211834_3seq TGTTTTCTACCAAGAACATGCAGTGTTGCAAG(+CAGTGT)GTACCATATCAAAATATTATATGGCTATATA NGG 10 8 17 8 24 0 100 5 10
    240605211832_1t87 AGCTGCCATGAGGATTCTGGGAATTGAATTTG(+CAGTGT)GATCCTCTGGACAAGTGACCAGTACTCTTAA NGG 10 8 17 8 24 0 100 5 10