Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211830_3x11 AGCAGTTAAGAGTACTGGTCACTTGTCCAGAG(+CAGTGT)GATCCAAATTCAATTCCCAGAATCCTCATGG NGG 10 8 17 8 24 0 100 5 10
    240605211828_mt7k GTGCTGTGTTCTGGAGAGATCTCACGTGGATG(+CAGTGT)GTACCGGTGCTCTGAGCACCACAGATGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605211826_3r60 TGTAGCTCACATCTGTGGTGCTCAGAGCACCG(+CAGTGT)GTACCATCCACGTGAGATCTCTCCAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605211825_g83y TCAAGGAACGTGCACCAAGAAGGCAGCTTGAG(+CAGTGT)GATCCTGACACTGAACTGAAGCGCGGGCATT NGG 10 8 17 8 24 0 100 5 10
    240605211824_y00q AACCCAATGCCCGCGCTTCAGTTCAGTGTCAG(+CAGTGT)GATCCTCAAGCTGCCTTCTTGGTGCACGTTC NGG 10 8 17 8 24 0 100 5 10
    240605211822_zr44 GCAACACCATCTGGTCCCTGTTTACTGTCCTG(+CAGTGT)GTACCTGCTGTTACTCCAGTTTATTTATTCC NGG 10 8 17 8 24 0 100 5 10
    240605211821_2xzq AGATGGGAATAAATAAACTGGAGTAACAGCAG(+CAGTGT)GTACCAGGACAGTAAACAGGGACCAGATGGT NGG 10 8 17 8 24 0 100 5 10
    240605211819_gcfc TTTTGAAGCTGCAAAGGATCTTTGGGGTTCTG(+CAGTGT)GATCCTCCAAGGAAGCAAGACACAGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605211818_nbs6 ATACCCTCTCTCTGTGTCTTGCTTCCTTGGAG(+CAGTGT)GATCCAGAACCCCAAAGATCCTTTGCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605211815_m5lj CTGAGCTACCATTCACAGACAGAGATACAGGG(+CAGTGT)GTACCCAGCCACAGTTACGACCAGCACCTCA NGG 10 8 17 8 24 0 100 5 10