Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211726_xunh GTGACAAAGAAACATTGCAGGTGTTCTCTGTG(+CAGTGT)GTACCTTGTCCTTGAGTTCTAACAGAACACG NGG 10 8 17 8 24 0 100 5 10
    240605211725_xi03 CCACCTGAGAGCAGGGACAACAGAACAGACAG(+CAGTGT)GTACCCATCAGTTCTGCTCCAGCAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605211724_l8bx CAGTAGCTTGCTTGCTGGAGCAGAACTGATGG(+CAGTGT)GTACCTGTCTGTTCTGTTGTCCCTGCTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605211722_ffse ATCCTGGGTCCTACCCAGGCAGTGGAAATGAG(+CAGTGT)GTACCATTTTTAAAGTACTTGTGTGAAATTA NGG 10 8 17 8 24 0 100 5 10
    240605211721_mte0 AAGAGTAATTTCACACAAGTACTTTAAAAATG(+CAGTGT)GTACCTCATTTCCACTGCCTGGGTAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605211720_5ada TGAATTTACACACCTGGATGGGAGACGTAGAG(+CAGTGT)GTACCACAACAGAGTTTCAGATTGGTGATAC NGG 10 8 17 8 24 0 100 5 10
    240605211718_j9ea TTCTGGTATCACCAATCTGAAACTCTGTTGTG(+CAGTGT)GTACCTCTACGTCTCCCATCCAGGTGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605211717_8x45 TTTCTCAATAAAAATAGGCCAGTCGCTAGAAG(+CAGTGT)GTACCATTCTGTGAAGACATTATTTTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605211716_cmzh CTAGGGCTTCAAAATAATGTCTTCACAGAATG(+CAGTGT)GTACCTTCTAGCGACTGGCCTATTTTTATTG NGG 10 8 17 8 24 0 100 5 10
    240605211714_lso8 GAGAAGGACATCCAGAGACTGTCCCACTTAGG(+CAGTGT)GATCCATTCCATCTACAGAGACCAAAACCAG NGG 10 8 17 8 24 0 100 5 10