Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211713_2u3z AGTGTCTGGTTTTGGTCTCTGTAGATGGAATG(+CAGTGT)GATCCCTAAGTGGGACAGTCTCTGGATGTCC NGG 10 8 17 8 24 0 100 5 10
    240605211711_4vw9 CCATCTTACTGCATAAAATAGCACTTTGACAG(+CAGTGT)GTACCGGCCCTCTTTATATGAAAGAAAATTT NGG 10 8 17 8 24 0 100 5 10
    240605211710_gf2w AATTTAAATTTTCTTTCATATAAAGAGGGCCG(+CAGTGT)GTACCTGTCAAAGTGCTATTTTATGCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240605211708_6c50 TTGTAGTTGCCCTGAACCAATCTCTATTCTAG(+CAGTGT)GTACCGTGACACCAGTTGAAACAGACCCACC NGG 10 8 17 8 24 0 100 5 10
    240605211707_hu4a AGGACGGTGGGTCTGTTTCAACTGGTGTCACG(+CAGTGT)GTACCTAGAATAGAGATTGGTTCAGGGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605211705_bh0s GCATTTGATGTTTGTAAATAAATCCACAGTAG(+CAGTGT)GATCCAAGCTGAAGAGGTGATACATGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605211704_taqg CTAGGCTGACCATGTATCACCTCTTCAGCTTG(+CAGTGT)GATCCTACTGTGGATTTATTTACAAACATCA NGG 10 8 17 8 24 0 100 5 10
    240605211703_us66 ATCCATGATGACTTCCAAGGACTGTGTGTTAG(+CAGTGT)GTACCCGAAAAAGCTCCAAAGCTTGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605211701_trea ATAGTCTGATTCAAGCTTTGGAGCTTTTTCGG(+CAGTGT)GTACCTAACACACAGTCCTTGGAAGTCATCA NGG 10 8 17 8 24 0 100 5 10
    240605211659_jns8 TGCTAAGCATGATCTAGAATAGAAGCCACTGG(+CAGTGT)GTACCCCTAACTATATTTAGTGATTTAGAAC NGG 10 8 17 8 24 0 100 5 10