Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213649_ysz0 GGCAAAGAACCCTGACAGTGGCAAACAAGCAG(+CAGTGT)GATCCCCCAGGCGCTGGCCCTAGAGCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605213647_t4g6 TATCAATTAGGCTCTAGGGCCAGCGCCTGGGG(+CAGTGT)GATCCTGCTTGTTTGCCACTGTCAGGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605213645_r1qi CACTGACAAGTGATATTAGCCATAAAGTGCAG(+CAGTGT)GTACCATGCCACACTCCACAGACCAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605213644_a4mv TTTCACCTCCTTGGTCTGTGGAGTGTGGCATG(+CAGTGT)GTACCTGCACTTTATGGCTAATATCACTTGT NGG 10 8 17 8 24 0 100 5 10
    240605213641_ey1m TGTTGCAGGGCTATAGGAAGACAAAGCCTGGG(+CAGTGT)GTACCCGCATCTGGTTTGGAACCCTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605213638_wh27 CAACAAAACTCAGGGTTCCAAACCAGATGCGG(+CAGTGT)GTACCCCAGGCTTTGTCTTCCTATAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605213637_x0g0 TTTTCTGTTTCACATCTTGTTTTTAAAAATAG(+CAGTGT)GATCCAAAACATCTCAAATCATCTCCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605213635_q5pv ATTCTGTTAAGGAGATGATTTGAGATGTTTTG(+CAGTGT)GATCCTATTTTTAAAAACAAGATGTGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605213634_x537 GTTTGTTATCATAGTTGGTCAATGCCTGGCAG(+CAGTGT)GTACCAGCATTTTGTCACTTGGGTCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605213632_pfxc TCCCTTGGTTTGACCCAAGTGACAAAATGCTG(+CAGTGT)GTACCTGCCAGGCATTGACCAACTATGATAA NGG 10 8 17 8 24 0 100 5 10