Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203221_48cx CATCCCAAGCACTGATGTGAAAGATGGGTGTG(+CAGTGT)GTACCATGGGCCTGGAACCCCAAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605203219_nh4v GCAACCCAATAATACTATCATAATCCGGACAG(+CAGTGT)GATCCTCCCCGACTGTGGAGAGAGACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605203218_tbyf TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+CAGTGT)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240605203217_1yn0 CACCAGCTTGACCCTCTCGATCTACAGACATG(+CAGTGT)GATCCGATCATTGCACACATTATAGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605203215_31ht TCTTGTGGAGCCTATAATGTGTGCAATGATCG(+CAGTGT)GATCCATGTCTGTAGATCGAGAGGGTCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605203214_impv GTCCCTCTGTCAGAATGAGCACACCTTACCTG(+CAGTGT)GATCCCAGATTTTCTGTACATGCGTTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605203210_u9co ACAAAGAACAAACGCATGTACAGAAAATCTGG(+CAGTGT)GATCCAGGTAAGGTGTGCTCATTCTGACAGA NGG 10 8 17 8 24 0 100 5 10
    240605203208_qvic CTACTGTGGCTGACATCCAAAGGTATGGGGAG(+CAGTGT)GATCCTTGCTAGGAAGACCTCAGTCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605203207_lehx TAAGATAAAAGGACTGAGGTCTTCCTAGCAAG(+CAGTGT)GATCCTCCCCATACCTTTGGATGTCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605203206_66t0 GCACGCTTTTCCTCCTGCTCCAAGGAGCTGAG(+CAGTGT)GATCCACCATATCCTCACCCTGACAGGGAGG NGG 10 8 17 8 24 0 100 5 10