Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203307_spxb TCAGATCCAGCATCAACACAAAGGCATGGTGG(+CAGTGT)GATCCTTGGGACCACTTCTTTCTAAACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605203306_fe7k CTCCTGCCTCCACAGTATCTGACTTCTCTGCG(+CAGTGT)GATCCAGTGATGCCCTCCCCCCTCCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605203303_y52a GAGTGGCTGAGGGAGGGGGGAGGGCATCACTG(+CAGTGT)GATCCGCAGAGAAGTCAGATACTGTGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605203301_46kl GCACATGAGCACTTCACGGGGTCTACCAGGTG(+CAGTGT)GTACCTGCAGCATCTTCCCTGTCTCTGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605203300_7zuo TCAGACTTGCAGAGACAGGGAAGATGCTGCAG(+CAGTGT)GTACCACCTGGTAGACCCCGTGAAGTGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605203259_80vo GGATGTTATACTGGCCTGGATTCTGCATAACG(+CAGTGT)GTACCACATGCTCCACCCCATCCTCCCTAGT NGG 10 8 17 8 24 0 100 5 10
    240605203257_zevt GGAGAACTAGGGAGGATGGGGTGGAGCATGTG(+CAGTGT)GTACCGTTATGCAGAATCCAGGCCAGTATAA NGG 10 8 17 8 24 0 100 5 10
    240605203255_4vb2 AACAACCAGGCTGTTAATGGGCAAACATCTGG(+CAGTGT)GTACCTCGGCCGAGTCAGGATGATTCATCAA NGG 10 8 17 8 24 0 100 5 10
    240605203254_pnp4 TTGCTTTGATGAATCATCCTGACTCGGCCGAG(+CAGTGT)GTACCCAGATGTTTGCCCATTAACAGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605203252_iaec AACGGGTCAAAACCTTCAGACAGGAAGTCTGG(+CAGTGT)GTACCACTCTGCAAGGTTACAATCACTCTGC NGG 10 8 17 8 24 0 100 5 10