Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203357_98mo GGCTTGGACCAAGGGAGGTGAGACCCCCTCAG(+CAGTGT)GTACCCTCCCCTTTCAGAATCCATCTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605203356_wnbn CCTCCTCCCCTTTTGTCATGAGAGCTAAGCTG(+CAGTGT)GATCCATAATCTCTGTCCACACTAAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605203355_6wme GTCAACAGCCTTTAGTGTGGACAGAGATTATG(+CAGTGT)GATCCAGCTTAGCTCTCATGACAAAAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605203353_ao13 GGCACTGAATAAAGAAACTGGAAAGTTCTCAG(+CAGTGT)GATCCTGTTCTAGTCCTGGGACCTGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605203352_8q7b GCCCAGGAAAGCAGGTCCCAGGACTAGAACAG(+CAGTGT)GATCCTGAGAACTTTCCAGTTTCTTTATTCA NGG 10 8 17 8 24 0 100 5 10
    240605203351_u3ll CCCTCTGTAGACCAGGCTAGCTTCAAATTCAG(+CAGTGT)GATCCCCAAGAAATACTCATCCCTGTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605203349_h1gz CTGTGGTGGCACAGGGATGAGTATTTCTTGGG(+CAGTGT)GATCCTGAATTTGAAGCTAGCCTGGTCTACA NGG 10 8 17 8 24 0 100 5 10
    240605203348_uz7g CCCTCTGTAGACCAGGCTGGCTTCAAATTCAG(+CAGTGT)GATCCCCAAGAATACTCATCCTTGAACCACC NGG 10 8 17 8 24 0 100 5 10
    240605203346_6ewg TCTGTGGTGGTTCAAGGATGAGTATTCTTGGG(+CAGTGT)GATCCTGAATTTGAAGCCAGCCTGGTCTACA NGG 10 8 17 8 24 0 100 5 10
    240605203344_he2u GCACACATGCAGAGGCCAGAGGACAATGCCAG(+CAGTGT)GATCCCACTCTATCAGTTTCTGCCTTATCCT NGG 10 8 17 8 24 0 100 5 10