Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203450_pgvc TGTAGAAGGCCCCTGAGTAATGGTAGCTGTGG(+CAGTGT)GTACCACCTGGGTGTTTATGAAAAACGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605203448_1fg8 ATTGCCCCCAGACGAGTCATTGCAGGCTCCTG(+CAGTGT)GATCCTAACAAAAAGAGCAGGGCTTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605203446_5h2a TGGTCCACAGGAAGCCCTGCTCTTTTTGTTAG(+CAGTGT)GATCCAGGAGCCTGCAATGACTCGTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605203445_6iao CCATGAGTACTGGAGATACAGGCATAAGACAG(+CAGTGT)GATCCATGCCTTCTTTCTACCATGGGAAACC NGG 10 8 17 8 24 0 100 5 10
    240605203444_tcj4 CCCTGGGTTTCCCATGGTAGAAAGAAGGCATG(+CAGTGT)GATCCTGTCTTATGCCTGTATCTCCAGTACT NGG 10 8 17 8 24 0 100 5 10
    240605203442_0urz GGGTGCTCCTTATGGGATCTTCTACCCAGTGG(+CAGTGT)GATCCTCCAGGTTCCTCTGTCCTGTCATCCC NGG 10 8 17 8 24 0 100 5 10
    240605203440_eqtm GGGGTGGGATGACAGGACAGAGGAACCTGGAG(+CAGTGT)GATCCCACTGGGTAGAAGATCCCATAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605203439_1n1x TAATGTCCTGACCAGTAACTTACCTCTTCCTG(+CAGTGT)GATCCATTCACACTGTTTCAGCCTTGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605203437_t8t5 CCCAGCACAACAAGGCTGAAACAGTGTGAATG(+CAGTGT)GATCCAGGAAGAGGTAAGTTACTGGTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605203436_3u58 CCCTGTGGGGGAAAGGTGGGGGTTGGGGCCTG(+CAGTGT)GATCCAGGGGAGGAGGCCATGGTGAAGTGGA NGG 10 8 17 8 24 0 100 5 10