Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203433_fkya AGTTTTCCACTTCACCATGGCCTCCTCCCCTG(+CAGTGT)GATCCAGGCCCCAACCCCCACCTTTCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605203430_9pm2 GCTTTGATACCTGGTTCAAGTGACCATGGGGG(+CAGTGT)GATCCCATAGTACCTGGAATGAGATTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605203429_cyrh ACGCTCCAGCAATCTCATTCCAGGTACTATGG(+CAGTGT)GATCCCCCCATGGTCACTTGAACCAGGTATC NGG 10 8 17 8 24 0 100 5 10
    240605203427_rfp7 GGGGGGAGGGGGCGCGCTCTGAGATAGAGCAG(+CAGTGT)GATCCTTCTGCCCTGGGGCCTGGTCCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605203426_zyrw CTGGTAGACTGGACCAGGCCCCAGGGCAGAAG(+CAGTGT)GATCCTGCTCTATCTCAGAGCGCGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605203424_elej ACAAAAAAAATAGTAGGATTGGACATTGGAGG(+CAGTGT)GTACCAAAAGCAGACTCCTAACAAGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605203423_vvcm TCCCTGTTCCCCTTGTTAGGAGTCTGCTTTTG(+CAGTGT)GTACCCTCCAATGTCCAATCCTACTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605203421_kg8g CGAGGAATCTGGTACTGGCATGCGGATTGGGG(+CAGTGT)GATCCTGAGTCCGTTTGAAAGACTGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605203419_fx6c CCTCCTCTGCCCAGTCTTTCAAACGGACTCAG(+CAGTGT)GATCCCCCAATCCGCATGCCAGTACCAGATT NGG 10 8 17 8 24 0 100 5 10
    240605203418_igoq TTCCTTGGCTTCGGTTTCTGCTGCTCTCATAG(+CAGTGT)GATCCCTTAAGAGAGGGGATGACTCGTCAAG NGG 10 8 17 8 24 0 100 5 10