Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203505_6m5q GCAGTACTAGAGGGGTTGTATGGAGCCCTGAG(+CAGTGT)GATCCACTAGCTTCTGCGTCTGGTGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240605203504_geg4 TTCATGTTGTTGGGAAAACTTAGCAGAAATCG(+CAGTGT)GTACCTCGGTCATTACAAAGAAGCACGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605203502_h2pp TCCTTTGAACGTGCTTCTTTGTAATGACCGAG(+CAGTGT)GTACCGATTTCTGCTAAGTTTTCCCAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605203501_qwi1 TGCAGGGAAAATACGAGGGGACTCCACTAATG(+CAGTGT)GTACCTTCATAAAACCACTCCCTGCTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605203459_3cqk GGCAAGAAAAAGCAGGGAGTGGTTTTATGAAG(+CAGTGT)GTACCATTAGTGGAGTCCCCTCGTATTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605203458_l7ug CGCCCACCAAGGCTCTAGCAGCAAGCGTGCAG(+CAGTGT)GTACCAATATGTCTGCTAGGGAAAATTAATA NGG 10 8 17 8 24 0 100 5 10
    240605203456_6y8f AGTTGTATTAATTTTCCCTAGCAGACATATTG(+CAGTGT)GTACCTGCACGCTTGCTGCTAGAGCCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605203454_bwa3 ATTAAAAGTGGGTAAGCTGCTGGCTGCAGATG(+CAGTGT)GTACCAGCTAAAGAGCATTGTACCTAAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605203453_b02m TTGCCACCCTTAGGTACAATGCTCTTTAGCTG(+CAGTGT)GTACCATCTGCAGCCAGCAGCTTACCCACTT NGG 10 8 17 8 24 0 100 5 10
    240605203451_1xc4 TAGAATGCACGTTTTTCATAAACACCCAGGTG(+CAGTGT)GTACCCACAGCTACCATTACTCAGGGGCCTT NGG 10 8 17 8 24 0 100 5 10