Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203251_ti9y AGCAGGCAGAGTGATTGTAACCTTGCAGAGTG(+CAGTGT)GTACCCAGACTTCCTGTCTGAAGGTTTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605203250_bq73 TAACCGAGGCTCCCCTCTTAGAGGAAGGCATG(+CAGTGT)GTACCGGCAGATTTTGGTAAGCAAAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605203248_lcs5 AGCAGCATTCCTTTGCTTACCAAAATCTGCCG(+CAGTGT)GTACCATGCCTTCCTCTAAGAGGGGAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605203247_0jm9 GGAAGCCAGGGCAGGGCAGAGCTCAGGTGATG(+CAGTGT)GTACCTTGTGACAAGCACAGGTTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605203246_l2uv GATGATCTGAAGAAACCTGTGCTTGTCACAAG(+CAGTGT)GTACCATCACCTGAGCTCTGCCCTGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605203244_gn38 CAACCATTTCCCCTTCCTGGATCCTCTGTCTG(+CAGTGT)GTACCAAGCAATATATTTTTTTATTTTACCC NGG 10 8 17 8 24 0 100 5 10
    240605203243_hcum TGCAAGGGTAAAATAAAAAAATATATTGCTTG(+CAGTGT)GTACCAGACAGAGGATCCAGGAAGGGGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605203242_fzxj TACCAACCTTGCTAGCCTGTGGGGACTTTAAG(+CAGTGT)GTACCGTGATGCATTTCATAAGGCTAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605203241_7lex CATATTCCCCTAGCCTTATGAAATGCATCACG(+CAGTGT)GTACCTTAAAGTCCCCACAGGCTAGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605203239_e28b ATTACCAACTGCTGTCTCTCTGCTGTGAAAAG(+CAGTGT)GTACCGTAATTCACGTGATCCATCTATACGT NGG 10 8 17 8 24 0 100 5 10