Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202128_600r GCGTTTGTTCAGAGTCTGTGGTGTAAGCAAAG(+CAGTGT)GATCCAATTTTAGCAGTGGTCTGTGGTTGAC NGG 10 8 17 8 24 0 100 5 10
    240605202126_d3eb AGTGGTGAAGAGCACTTGTTAGTTTTCCAAAG(+CAGTGT)GATCCAGGTTTGATATCCAAGACCTACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605202125_jwhl AGGCTCTCTGTAGGTCTTGGATATCAAACCTG(+CAGTGT)GATCCTTTGGAAAACTAACAAGTGCTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605202122_j6g9 GAGCCCGCGCGCCCCGCTGCCCTCCTCGCCCG(+CAGTGT)GATCCCTCTCCTGCCCTCTGACTGGAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605202121_chss GTGTAGAGACTCCAGTCAGAGGGCAGGAGAGG(+CAGTGT)GATCCGGGCGAGGAGGGCAGCGGGGCGCGCG NGG 10 8 17 8 24 0 100 5 10
    240605202119_6946 CACCCTGGAGCAGCTAGGGGTTCACGGACCTG(+CAGTGT)GATCCTACACAGATCCCAGGTTCCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605202118_9c4c GATGGGAGGAGGGGAACCTGGGATCTGTGTAG(+CAGTGT)GATCCAGGTCCGTGAACCCCTAGCTGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605202117_1e0t CTCTGGGCTCAACAGCCCTGGAGAAGGACGAG(+CAGTGT)GATCCCCATCCTCCAGCAAGTCCCAACACAT NGG 10 8 17 8 24 0 100 5 10
    240605202115_v38z CCAATATGTGTTGGGACTTGCTGGAGGATGGG(+CAGTGT)GATCCTCGTCCTTCTCCAGGGCTGTTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605202114_7p7r GGGGCACACACACGGACACACACACACACACG(+CAGTGT)GATCCAAGGGAGGGGGCTGAAAGATATGGCT NGG 10 8 17 8 24 0 100 5 10