Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202055_mkrr CTCGTTCCAGGTCAGAATTCCTGCCCACAGAG(+CAGTGT)GATCCTCTACAGTGCCCCCAGGAAGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605202052_47h6 AAGGGTCAGGGCCACATGAACACAGAACTAAG(+CAGTGT)GTACCTGTCAGTAGGACAGGGCATGCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605202050_f29s CAGCTGCTCTGCATGCCCTGTCCTACTGACAG(+CAGTGT)GTACCTTAGTTCTGTGTTCATGTGGCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605202049_cjr5 ATGGGAACCGACCTGCTAGGGAAGTGGGGGAG(+CAGTGT)GATCCGTACCTAGGAGGCCCTTCCTGACTGC NGG 10 8 17 8 24 0 100 5 10
    240605202048_hsib AAGCAGCAGTCAGGAAGGGCCTCCTAGGTACG(+CAGTGT)GATCCTCCCCCACTTCCCTAGCAGGTCGGTT NGG 10 8 17 8 24 0 100 5 10
    240605202046_9fcp AAAGGACCAAGTATTAGTGCTGTTTTTCAAGG(+CAGTGT)GATCCAGGGAACTTGAGAGAGAAAGCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605202044_jp2b ACACACACAGGCTTTCTCTCTCAAGTTCCCTG(+CAGTGT)GATCCCTTGAAAAACAGCACTAATACTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605202042_5nj4 ACATCCCTGGCCCATGGCAGGGCTTACAGAGG(+CAGTGT)GATCCTGATGCAGTGCAGGGCAATTCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605202040_ecwy ACGGAGAACAGAATTGCCCTGCACTGCATCAG(+CAGTGT)GATCCCTCTGTAAGCCCTGCCATGGGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605202038_ozpu CACTATCCAGGAGAGCCAGTATGGTTTGTTGG(+CAGTGT)GTACCATCTGTAGCAAATAACATCCAGCTGT NGG 10 8 17 8 24 0 100 5 10