Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202000_pxmx ATTTGCATCTGCCAATGGATCTTGGTCCCTTG(+CAGTGT)GATCCACTGACTGAGAAAAGTAATGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605201958_79ku AAAAAGCAGCGGCAGACAGGATTTTTGTCCAG(+CAGTGT)GATCCACTATCCAGCTTTAAGATGAGTCATT NGG 10 8 17 8 24 0 100 5 10
    240605201957_7fmd GGTCGAATGACTCATCTTAAAGCTGGATAGTG(+CAGTGT)GATCCTGGACAAAAATCCTGTCTGCCGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605201955_upr4 ATCTCTTTCGTGCAAACCACATTTCTACACTG(+CAGTGT)GTACCTCCTAAGTCCAGAAGTTGGCCTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605201954_f7j9 TGGGCACGGAGGCCAACTTCTGGACTTAGGAG(+CAGTGT)GTACCAGTGTAGAAATGTGGTTTGCACGAAA NGG 10 8 17 8 24 0 100 5 10
    240605201952_qe85 GGTGTCCAGAGTGCCTGCAGACAGTGTGCCCG(+CAGTGT)GTACCTCTGTCTACCTTTTGTAACGTTACCG NGG 10 8 17 8 24 0 100 5 10
    240605201951_vnrq CCAGGCGGTAACGTTACAAAAGGTAGACAGAG(+CAGTGT)GTACCGGGCACACTGTCTGCAGGCACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605201949_le6o TACCACCCACCACGTATTACCCAAATACTCAG(+CAGTGT)GATCCTACCAGCTACTGAGGCCACTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605201948_84yf CCCATGCACACAGTGGCCTCAGTAGCTGGTAG(+CAGTGT)GATCCTGAGTATTTGGGTAATACGTGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605201946_fams CAGCACACTGAGAACATCTTAGTGAGTACACG(+CAGTGT)GTACCTTAACTTGATATTTTTCAGCAAATGT NGG 10 8 17 8 24 0 100 5 10