Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202111_df37 CTCTCAGCCATATCTTTCAGCCCCCTCCCTTG(+CAGTGT)GATCCGTGTGTGTGTGTGTGTCCGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605202110_jlam AGCCCCTGGCACTTGAGGTGTGTGACCATTGG(+CAGTGT)GATCCGGATAGCCGCAGCGGGGCTGAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605202107_hiaf CAGAGAGACCTCAGCCCCGCTGCGGCTATCCG(+CAGTGT)GATCCCAATGGTCACACACCTCAAGTGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605202106_sbaa GCCTCTGCCCAGCTGAAAAGAGCAGCTTTCCG(+CAGTGT)GATCCCTTTTCAGACATGCACTGCTAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605202104_0w1l TCCTAACAACTAGCAGTGCATGTCTGAAAAGG(+CAGTGT)GATCCGGAAAGCTGCTCTTTTCAGCTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605202102_i0e1 AGGCTTTCCTTATAGGCGTCATCTTCAGTGAG(+CAGTGT)GTACCCAAGCACTCCAGGGTACAGCGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605202101_hshs CATAGCCAGGCGCTGTACCCTGGAGTGCTTGG(+CAGTGT)GTACCTCACTGAAGATGACGCCTATAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605202059_fur4 CATTTTGGGAAGAGTCTCCGCAAACACGGCAG(+CAGTGT)GATCCGGAAGGCTTTGAATTAGTTCCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605202057_y9iu GAGATTCTAGGGAACTAATTCAAAGCCTTCCG(+CAGTGT)GATCCTGCCGTGTTTGCGGAGACTCTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605202056_e0ik AACTTCTGATTCTTCCTGGGGGCACTGTAGAG(+CAGTGT)GATCCTCTGTGGGCAGGAATTCTGACCTGGA NGG 10 8 17 8 24 0 100 5 10