Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201929_3l5b CAGATAGCACTCTGCTCTGGCGCTCTGACATG(+CAGTGT)GTACCACACTGCTGCATGTGGAGGTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605201928_82gy CATGAAAGAGTTATTAGCCACCAAGTATGCTG(+CAGTGT)GTACCTATAATCCCAGAACACAGAAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605201926_2gs9 CTGCCTCAGCCTTCTGTGTTCTGGGATTATAG(+CAGTGT)GTACCAGCATACTTGGTGGCTAATAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240605201925_z4x2 GCATGAAGTTATCTGTGAGATGAGGGTAGGAG(+CAGTGT)GATCCTGGGAGTCAGGACCACCCAGAACCAA NGG 10 8 17 8 24 0 100 5 10
    240605201922_io62 GTGGGTTGGTTCTGGGTGGTCCTGACTCCCAG(+CAGTGT)GATCCTCCTACCCTCATCTCACAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240605201920_4ojg CCTGGAGGGCCTGGGCTGGAAATTTGCCCCTG(+CAGTGT)GATCCAGAACCCCATTCGTTTTACAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605201918_vqzl CCTATCCCAACTGTAAAACGAATGGGGTTCTG(+CAGTGT)GATCCAGGGGCAAATTTCCAGCCCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605201917_cmir TGCGTTCTTGCCATGAGCGAGCTGACTATCAG(+CAGTGT)GTACCTGTTCTCGCTCTGTCTGGGAACTGTG NGG 10 8 17 8 24 0 100 5 10
    240605201916_fwha TGTGACACAGTTCCCAGACAGAGCGAGAACAG(+CAGTGT)GTACCTGATAGTCAGCTCGCTCATGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605201914_bwtd GGCTAAGGATGGCCCTAAACATCCATCCTTCG(+CAGTGT)GATCCTCCTGCCTCCTTTTCTCTAGTGCGGG NGG 10 8 17 8 24 0 100 5 10