Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203546_5l0k TCCTCACACCCAGCCTTGGCCTCTTCTCTGGG(+CAGTGT)GATCCCCTCCAGCTAGGCCTGCTTCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605203544_86hb AACATGGAGGAGGAAGATGCCAGCTGTAAGGG(+CAGTGT)GATCCAGACAGATCCACAGGTCAGAGAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605203543_vq55 CCTCTAAGCTCTCTGACCTGTGGATCTGTCTG(+CAGTGT)GATCCCCTTACAGCTGGCATCTTCCTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605203541_u6kx GGTGCACAACCACGATCAAGTCAGGACAGGTG(+CAGTGT)GATCCACAGCCAAGCTCCTGTTCCCCCATGC NGG 10 8 17 8 24 0 100 5 10
    240605203540_8twb TGCAGGCATGGGGGAACAGGAGCTTGGCTGTG(+CAGTGT)GATCCACCTGTCCTGACTTGATCGTGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605203538_iiuu ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTATGCAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605203537_trsp TACTTAAAACTGCATACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605203535_yhjs TACATCCCCAGCCAGCCTTTGTCTTTCTGTGG(+CAGTGT)GTACCTGTGGTGCTGGGACCCGAACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605203533_65a6 TATTCCTGAGGGTTCGGGTCCCAGCACCACAG(+CAGTGT)GTACCCACAGAAAGACAAAGGCTGGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605203532_jxg7 ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCTCTTCTGGTGTGTCTGAAGACAGTGA NGG 10 8 17 8 24 0 100 5 10