Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201807_bkbp GCCCAGAATATCTAAGTTTTGAAACATAAACG(+CAGTGT)GTACCTTCCTTAAATAAGAGCTGAAGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605201806_ueha GCTATTTCTTGCTCCAGGAGCATGGCTACCAG(+CAGTGT)GATCCCCAATATTCAGCATCAACTGTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605201804_66vz CCTGGTGAAAACAGTTGATGCTGAATATTGGG(+CAGTGT)GATCCTGGTAGCCATGCTCCTGGAGCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605201802_3i1s CTGTAGCTGTCTTCAGAGACATTAGGTCAAAG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605201801_i1so ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCTTTGACCTAATGTCTCTGAAGACAGC NGG 10 8 17 8 24 0 100 5 10
    240605201759_76xi AGCTCACAACCATTTGTAACTCCAATTTTAGG(+CAGTGT)GATCCAGTGCCTAACTCTGGCTTCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605201758_2cod CTGGTGCCTGTGGAAGCCAGAGTTAGGCACTG(+CAGTGT)GATCCCTAAAATTGGAGTTACAAATGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605201756_ykc6 TGATACCAAGACCTAACAAACCCCGTGCCTGG(+CAGTGT)GTACCTCTTTTAAAGCTCTGCACCCCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605201755_7j8e CCTTGAAGAAGGGGTGCAGAGCTTTAAAAGAG(+CAGTGT)GTACCCAGGCACGGGGTTTGTTAGGTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605201753_x1kp ACAGCCCATCTGCCACAAGAGCTTCCTGATTG(+CAGTGT)GTACCACTGTGAACCGTCCCTCCCCCCTCCA NGG 10 8 17 8 24 0 100 5 10