Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201659_mw4p GATACCATCTGAAAGCTCTCTGTGGTCCAAGG(+CAGTGT)GATCCAAAGGTTGCAAAGAGTCTTGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605201657_234q CTCTCTCTTCCAGGAGAGGTGGGTGGTCTTGG(+CAGTGT)GATCCGCCCCTCCCCCGCCCCCAGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605201655_j5bb TGAGACAGGGTTCTGGGGGCGGGGGAGGGGCG(+CAGTGT)GATCCCAAGACCACCCACCTCTCCTGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605201653_nja0 AAATAAATATATATGTTAAATAAAAGGCCCAG(+CAGTGT)GTACCAAAGAGCAAAATTTAATGAAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605201651_1rts CAGTTCTGGCTTTCATTAAATTTTGCTCTTTG(+CAGTGT)GTACCTGGGCCTTTTATTTAACATATATATT NGG 10 8 17 8 24 0 100 5 10
    240605201650_1ek2 AGGCTCCACAGAGAGTCCTACTCCACCATGGG(+CAGTGT)GATCCAGTATAGACATCAAGCAAAGGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605201649_04b0 CTTGCCTTTTCCTTTGCTTGATGTCTATACTG(+CAGTGT)GATCCCCATGGTGGAGTAGGACTCTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605201647_m2nd GTATGCGTTCATGGTGGAACACAACCACATCG(+CAGTGT)GTACCTACTTCAGAACAATTATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605201646_zn0s GTTTTTTTTCTCAGATAATTGTTCTGAAGTAG(+CAGTGT)GTACCGATGTGGTTGTGTTCCACCATGAACG NGG 10 8 17 8 24 0 100 5 10
    240605201644_8qbz AGCTTCAGGACTTGTTCCCCCTCTGCCGGTGG(+CAGTGT)GATCCTGCCTGAAACCTTCTGCGCAGAAGAA NGG 10 8 17 8 24 0 100 5 10