Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201610_4epy ATTAAGACTCCTTCAGAAAAGCTAGTACTTTG(+CAGTGT)GTACCTGATTTAGTGTAATCAATTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240605201608_rb17 GGGAGAGGATGACTCTCTTGATGGTGTATGGG(+CAGTGT)GATCCACAACTTGAGGGTCATTTGGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605201607_cy8u CTATTGTGCTGCCAAATGACCCTCAAGTTGTG(+CAGTGT)GATCCCCATACACCATCAAGAGAGTCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605201605_a581 ATGACTTCAGGCCTTTCCCAGCCTCCTCCCGG(+CAGTGT)GATCCAGGAAGGCAGCAGGATATTTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605201603_4bsl CCACATCACACAAATATCCTGCTGCCTTCCTG(+CAGTGT)GATCCCGGGAGGAGGCTGGGAAAGGCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605201601_of00 GCAACTGACAGAAGTGTCAGCCTGGGCAGCAG(+CAGTGT)GATCCCTCGGTTCTGTGGTGTCCCCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605201559_v44j CGTGTTGCCTGGGGGACACCACAGAACCGAGG(+CAGTGT)GATCCTGCTGCCCAGGCTGACACTTCTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605201557_zk4f GATTTGGAAGTGGGTGCACTGCCACACCTGTG(+CAGTGT)GTACCAGCACTTGAGAAGCTGAGGCAGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605201555_2zkd CCGGTCTTACTGCCTCAGCTTCTCAAGTGCTG(+CAGTGT)GTACCACAGGTGTGGCAGTGCACCCACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605201553_0kek ATTAGATCTATGTAGATGTATCATCTTAAGGG(+CAGTGT)GATCCTGCCGCCTCTGACAGCCATATCTCTT NGG 10 8 17 8 24 0 100 5 10