Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203334_i0cs CTGGTACACGAAGAGGTGAAAAGAAGGTGCCG(+CAGTGT)GATCCCCTGGAATGAGAGTTACTGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605203333_27hp TTTGAATGTGCACACCATGCGTGTGCCTGTTG(+CAGTGT)GATCCCCTGGAACTGGGCTTGCGGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605203330_ccrk GCTCACAACCATCCGCAAGCCCAGTTCCAGGG(+CAGTGT)GATCCAACAGGCACACGCATGGTGTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240605203329_ln0e AAGGGGCCCCCCACTCTACCCTGGGGAACTTG(+CAGTGT)GATCCTCTTCAGAACCCTACCATGTTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605203328_5r5q ACACACAGACAACATGGTAGGGTTCTGAAGAG(+CAGTGT)GATCCAAGTTCCCCAGGGTAGAGTGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605203326_64xm AATCCCAGCACTTAAGGGGGTGGAGGTAAGAG(+CAGTGT)GATCCTGAGTTCAAGGCCAGCCTGGGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605203325_ushp TAATGTTAAGCCCAGGCTGGCCTTGAACTCAG(+CAGTGT)GATCCTCTTACCTCCACCCCCTTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605203323_svct GAGGCTAATACCAAACCTATAGCTACTCCTGG(+CAGTGT)GTACCCTGCCTCCAGGTGATCACAGCCATCC NGG 10 8 17 8 24 0 100 5 10
    240605203321_pbx8 CGCAAGGATGGCTGTGATCACCTGGAGGCAGG(+CAGTGT)GTACCCAGGAGTAGCTATAGGTTTGGTATTA NGG 10 8 17 8 24 0 100 5 10
    240605203318_md4b AGAGCACCCCATCCAACAGGCTTCTTGCCCTG(+CAGTGT)GTACCATGGAAGCACAGTTCTGGGTTTCATG NGG 10 8 17 8 24 0 100 5 10