Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201511_ph42 AAATACCTTACAATACATTAGATTCCCAAAAG(+CAGTGT)GTACCAAAAAGTACAGTAAAATTAACACTTC NGG 10 8 17 8 24 0 100 5 10
    240605201506_k3np GTAGGGGTGGCTAACAAGCAGGAATAGGAAGG(+CAGTGT)GATCCTTGGTAGCTTGTTTTTTGTCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605201504_626o AGATCCCAACAGACAAAAAACAAGCTACCAAG(+CAGTGT)GATCCCTTCCTATTCCTGCTTGTTAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240605201503_uj87 AAGTCACAGGTTGGTAGGTGTCATTGTCATTG(+CAGTGT)GATCCTAGCTGACAGACAGATGAGGGGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605201501_vgf9 AGTGGATTCCCCCTCATCTGTCTGTCAGCTAG(+CAGTGT)GATCCAATGACAATGACACCTACCAACCTGT NGG 10 8 17 8 24 0 100 5 10
    240605201459_ld5u AGCCTACCCCTGACACCGCCTGCAGGGCCAAG(+CAGTGT)GATCCAAAGCCTGGAAAGGAGAACCAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605201458_s9ym CAGGGATGTTTGGTTCTCCTTTCCAGGCTTTG(+CAGTGT)GATCCTTGGCCCTGCAGGCGGTGTCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605201453_0mon GAATAATTTTTGGGACGTGGAAATTGCTAGAG(+CAGTGT)GTACCTTTTCTGTAACAATGAGAAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605201451_nj78 TCCTAGAAGCTCTTCTCATTGTTACAGAAAAG(+CAGTGT)GTACCTCTAGCAATTTCCACGTCCCAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605201449_zxzl GGCATTTTCCTAATTAGTGATTGATAGGGGAG(+CAGTGT)GATCCAGCCATTTGTGGGTGGTGCCATCCCT NGG 10 8 17 8 24 0 100 5 10