Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201413_2jjo CTGGTCCCACGGGCAGGAGACAGCTGTGAGTG(+CAGTGT)GATCCAGGTGCCCCCAAAGAGCGGCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605201411_ck5i GAAAGGAGGGGGGCCACAGGCAAGAAGACAGG(+CAGTGT)GTACCCACGCATAGACTTTCCATCTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605201409_5b9y GCGTTGTTGGGAGATGGAAAGTCTATGCGTGG(+CAGTGT)GTACCCTGTCTTCTTGCCTGTGGCCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605201408_fq0e GCCAAGTGGTATTTTGGAAACAGGGACATCGG(+CAGTGT)GTACCCTACAGAGTGTAGACAAACACGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605201406_haqj GGGTCAAAACGTGTTTGTCTACACTCTGTAGG(+CAGTGT)GTACCCGATGTCCCTGTTTCCAAAATACCAC NGG 10 8 17 8 24 0 100 5 10
    240605201404_wrnu GCCAAGTGGTATTTTGGAAACAGGGACATCGG(+CAGTGT)GTACCCTACAGAGTGTAGACAAACACGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605201403_pg8z GGGTCAAAACGTGTTTGTCTACACTCTGTAGG(+CAGTGT)GTACCCGATGTCCCTGTTTCCAAAATACCAC NGG 10 8 17 8 24 0 100 5 10
    240605201401_1xpj TCCCTCCGCAGATCTAGTATAAGGGCAGCCTG(+CAGTGT)GTACCCTTCTTCTTCCACAGAAGCCAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605201359_gre6 TAAGGTAACCTGGCTTCTGTGGAAGAAGAAGG(+CAGTGT)GTACCAGGCTGCCCTTATACTAGATCTGCGG NGG 10 8 17 8 24 0 100 5 10
    240605201356_js6l TCCCAGAATAAACAATCCAGGAAGGCCAGCTG(+CAGTGT)GATCCAAGGGACTCAACGACCTCTCCAGGCA NGG 10 8 17 8 24 0 100 5 10