Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201447_ifxh AGCCAAGGGATGGCACCACCCACAAATGGCTG(+CAGTGT)GATCCTCCCCTATCAATCACTAATTAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605201446_moxt GCCACAGATGGTACATCTAGTGGTCCAGATAG(+CAGTGT)GATCCCTCCTGAAATCTCCTACTTCTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605201444_pjct GTGGCAGACAAGAAGTAGGAGATTTCAGGAGG(+CAGTGT)GATCCTATCTGGACCACTAGATGTACCATCT NGG 10 8 17 8 24 0 100 5 10
    240605201443_jeu5 TCTACCAAAAACAGGGCTAAGGGCTGGAGATG(+CAGTGT)GATCCCCGTTCCTTGTCCAGCCTCCTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605201441_49t2 CAGCGGTAGCAGGAGGCTGGACAAGGAACGGG(+CAGTGT)GATCCATCTCCAGCCCTTAGCCCTGTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605201438_t4tx GTGCATATGCTGACGCCTGAGAACAGACCCAG(+CAGTGT)GTACCCGTGGCTGAGCTGAGCTTCCTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605201436_jxfe GGGAAAGCAGAGGAAGCTCAGCTCAGCCACGG(+CAGTGT)GTACCTGGGTCTGTTCTCAGGCGTCAGCATA NGG 10 8 17 8 24 0 100 5 10
    240605201434_aad0 GACATGCCACCCACCGCACTTGGCTTTATCAG(+CAGTGT)GATCCATTTGTTGGTAGAAGCAAAAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605201433_koqk CCAAGAGCTGCTTTTGCTTCTACCAACAAATG(+CAGTGT)GATCCTGATAAAGCCAAGTGCGGTGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240605201432_h032 CCTTTTCTGTGTGCTGTACATCCCAGTCAGTG(+CAGTGT)GTACCTAGCCCAGTTTCTCTCTGGCTGGCAC NGG 10 8 17 8 24 0 100 5 10