Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201531_411k AGTCCCCTGAGTAAAGAACACAGCTCAGGAAG(+CAGTGT)GATCCAGGGAACCCTTTCTCAGAGCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605201529_4nnx TGGTGGAAACATCCTTCCAACAAGTGTAGTGG(+CAGTGT)GATCCCGGGGCCCAGGGCAGGCCGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605201526_g3ez GCAGGCCAGCCTCGGCCTGCCCTGGGCCCCGG(+CAGTGT)GATCCCACTACACTTGTTGGAAGGATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605201524_m0uu ATGGACTCCGAGGGCGTGGGGTGGGCTCCAAG(+CAGTGT)GATCCTGGGCATCCGGATGGCATCGGCTACC NGG 10 8 17 8 24 0 100 5 10
    240605201521_gv8a TATGTGGTAGCCGATGCCATCCGGATGCCCAG(+CAGTGT)GATCCTTGGAGCCCACCCCACGCCCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240605201520_rz1c TTAATTCCAAGCCACACATGATGGTGGCACAG(+CAGTGT)GATCCTAACCCCAGTGCTTGGAAGGCTACGG NGG 10 8 17 8 24 0 100 5 10
    240605201517_gql7 TGTAGCCGTAGCCTTCCAAGCACTGGGGTTAG(+CAGTGT)GATCCTGTGCCACCATCATGTGTGGCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605201515_cr4a GGAGGGCTCCAGCGGGCAATGGGATGGATAGG(+CAGTGT)GTACCTGCTCCCGAAGCTGTGGCAAAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605201514_v5fq CTAGCAAGACTTTGCCACAGCTTCGGGAGCAG(+CAGTGT)GTACCCTATCCATCCCATTGCCCGCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605201512_oeeh TAATGGAAGTGTTAATTTTACTGTACTTTTTG(+CAGTGT)GTACCTTTTGGGAATCTAATGTATTGTAAGG NGG 10 8 17 8 24 0 100 5 10