Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201752_n9k3 CTGTCTGGAGGGGGGAGGGACGGTTCACAGTG(+CAGTGT)GTACCAATCAGGAAGCTCTTGTGGCAGATGG NGG 10 8 17 8 24 0 100 5 10
    240605201750_pb7g GTGCTTCAGGGTGGGCCCTATTTGGGCTTGAG(+CAGTGT)GATCCCGTGGGGGCGGGGCTTCACCCCGACT NGG 10 8 17 8 24 0 100 5 10
    240605201748_c25p CACCCAGTCGGGGTGAAGCCCCGCCCCCACGG(+CAGTGT)GATCCTCAAGCCCAAATAGGGCCCACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605201746_y1bz CTCTAGGAATCTCTATGGGGACTGCTAGGAAG(+CAGTGT)GATCCTGGGCATGACAGCCTCGTATGATAGC NGG 10 8 17 8 24 0 100 5 10
    240605201744_6g4d TGCAGGCTATCATACGAGGCTGTCATGCCCAG(+CAGTGT)GATCCTTCCTAGCAGTCCCCATAGAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240605201742_mte4 ATAGATCCTAAGATCCAGCAAGCTTGGCTCAG(+CAGTGT)GATCCTGTTGGGGAGCAGCCACCTGCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605201741_m9w9 CTGGGATAAGGCAGGTGGCTGCTCCCCAACAG(+CAGTGT)GATCCTGAGCCAAGCTTGCTGGATCTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240605201740_oj91 GAGCTGAAGCCCCTTAGGAGGTGCTGGCTCAG(+CAGTGT)GTACCTCCCTGCGGGATAGGAAGGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605201738_7u22 CCTGGTCACTCCCCTTCCTATCCCGCAGGGAG(+CAGTGT)GTACCTGAGCCAGCACCTCCTAAGGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605201737_e3ea TTGTATAGGTTGTGTGCAGGGTCGGGAGGTTG(+CAGTGT)GATCCCCTGGGTTGAGAGATGTGTTCTGTGA NGG 10 8 17 8 24 0 100 5 10