Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201116_v0qd CTGCCCGTACTCTGTGTGTTTTAGAACTGGAG(+CAGTGT)GATCCTGTGAACTGGCCACATGCCACACACA NGG 10 8 17 8 24 0 100 5 10
    240605201114_j5zt AATGAGCTACCCAACTTCTACCACATTCCTGG(+CAGTGT)GTACCCAACTGTGTTGTTACTCTGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240605201112_w8ew AAACTGTGGGTTCAGAGTAACAACACAGTTGG(+CAGTGT)GTACCCAGGAATGTGGTAGAAGTTGGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605201111_wrd5 GAAGAAGCCCAAGAAAAAAGCAGGGTTATCTG(+CAGTGT)GTACCTATCCTGGGCAGAATCACAGGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605201110_je4z ATCAGTACAACCTGTGATTCTGCCCAGGATAG(+CAGTGT)GTACCAGATAACCCTGCTTTTTTCTTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605201109_dku1 CACACGTTAAGATGCCTTTCTCGCCTCTGAAG(+CAGTGT)GATCCTGAGTCACTGCCCTCATTTACTATGA NGG 10 8 17 8 24 0 100 5 10
    240605201108_s74n GGGATTCATAGTAAATGAGGGCAGTGACTCAG(+CAGTGT)GATCCTTCAGAGGCGAGAAAGGCATCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605201106_1fk0 TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+CAGTGT)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605201104_t0qa TCTCTGTAGACCAGGCTGGCCTCGAACTCAGG(+CAGTGT)GATCCACCTAGCTCTGTCTCTGAGTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605201103_fh7q GTATTGTTTGTCAGCTCAGAAAGAATCAGGTG(+CAGTGT)GTACCCAAGTGCGGGGTCTTCCTAACGTTGA NGG 10 8 17 8 24 0 100 5 10