Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201044_zkuq CCTCCGCAGCCCCTTCAGTCTCAAGCAGTCAG(+CAGTGT)GATCCCAGAGGGGCACCCCAACCTTGATTCT NGG 10 8 17 8 24 0 100 5 10
    240605201043_7cre TCTATCCATCACTTGGTAAATCGGAAAGGATG(+CAGTGT)GATCCCGAGATCATTGTGATAGTGTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240605201041_m9ea ATTTTTGATAAACACTATCACAATGATCTCGG(+CAGTGT)GATCCATCCTTTCCGATTTACCAAGTGATGG NGG 10 8 17 8 24 0 100 5 10
    240605201040_l3xf CAAGGACTCAGAACAACATGGCTTTTTGATCG(+CAGTGT)GTACCAACCGACCTACATCAATTAGTTATTG NGG 10 8 17 8 24 0 100 5 10
    240605201039_p5rn AAGTCCAATAACTAATTGATGTAGGTCGGTTG(+CAGTGT)GTACCGATCAAAAAGCCATGTTGTTCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605201037_stno CCTTCTGTGGAAAAAAGTTTGAGTTTTGGTAG(+CAGTGT)GTACCCTTGCAAGGAATTCCAACCTAGATGT NGG 10 8 17 8 24 0 100 5 10
    240605201035_05u1 TTCTAACATCTAGGTTGGAATTCCTTGCAAGG(+CAGTGT)GTACCTACCAAAACTCAAACTTTTTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240605201034_lxp3 CTAACATTTAAGTGTTGGAAAGCTGAAAACAG(+CAGTGT)GTACCTGAGAGTAACTAGCCAATAATACCCC NGG 10 8 17 8 24 0 100 5 10
    240605201032_17r2 ACTCAGGGGTATTATTGGCTAGTTACTCTCAG(+CAGTGT)GTACCTGTTTTCAGCTTTCCAACACTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605201031_e721 AAAATGAATTGATGTCATGTTTAGAAGAATAG(+CAGTGT)GTACCATGGATGAAAATGAAATGCACATAAG NGG 10 8 17 8 24 0 100 5 10