Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201058_e756 GTTGATCGTTTGTCTACACTCAATAGTCCTTG(+CAGTGT)GTACCAGTCCTATGCACAGCAGGTAATGGGC NGG 10 8 17 8 24 0 100 5 10
    240605201056_uri1 TCTGGGTTCAAAACATAAACAAAATATACCCG(+CAGTGT)GTACCAAAATAAAATAGTACAAACCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240605201055_kyvn AGCTAGTATTTGGTTTGTACTATTTTATTTTG(+CAGTGT)GTACCGGGTATATTTTGTTTATGTTTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605201053_ljju TTTAATTTCTAAGAATGTGCATGTGTCTGTGG(+CAGTGT)GTACCACTGCCCAATACAGCCAGAAAATGGT NGG 10 8 17 8 24 0 100 5 10
    240605201052_qzjs CTCATACCATTTTCTGGCTGTATTGGGCAGTG(+CAGTGT)GTACCCACAGACACATGCACATTCTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605201051_inua GTACCCTTCCTTTACCCCCAGTGGCTGCTAAG(+CAGTGT)GTACCTTTGAAACACAAATCAGATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605201050_ib74 TGTAATATAAATATCTGATTTGTGTTTCAAAG(+CAGTGT)GTACCTTAGCAGCCACTGGGGGTAAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605201048_ue64 GGTTCTCTCTCACGGATTACCGCTCCTGATAG(+CAGTGT)GTACCTGACAGGCTCATTCTATATCCCAATT NGG 10 8 17 8 24 0 100 5 10
    240605201047_dnr3 CTCAAAATTGGGATATAGAATGAGCCTGTCAG(+CAGTGT)GTACCTATCAGGAGCGGTAATCCGTGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605201046_lr7f GGACTAGAATCAAGGTTGGGGTGCCCCTCTGG(+CAGTGT)GATCCTGACTGCTTGAGACTGAAGGGGCTGC NGG 10 8 17 8 24 0 100 5 10