Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200956_5cxi ATTTCGCCACATGGCTAATTACCTGTGCTCAG(+CAGTGT)GTACCACTGCCACACGTTCCCAAGTGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605200954_v3mp CTCCTCTTTGGGGGTGCTGCATTCAAGCCTGG(+CAGTGT)GTACCTCCAAGAAGAGATCACAGCCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605200952_2yj1 AACTACCAACAGGCTGTGATCTCTTCTTGGAG(+CAGTGT)GTACCCAGGCTTGAATGCAGCACCCCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605200951_03v4 AATGTGCCCATGCCGACCGTGTTAGTCTGTAG(+CAGTGT)GTACCGCACTCACCCCATACCTTGGGCGTCT NGG 10 8 17 8 24 0 100 5 10
    240605200950_9zac GATGCAGACGCCCAAGGTATGGGGTGAGTGCG(+CAGTGT)GTACCTACAGACTAACACGGTCGGCATGGGC NGG 10 8 17 8 24 0 100 5 10
    240605200949_r0ae GGGAGGGGCACGAAGTGGGGAACGGGACACAG(+CAGTGT)GATCCTAAACTCCAAGGGGACTGTCCACCTA NGG 10 8 17 8 24 0 100 5 10
    240605200947_4ntp GTCTGTAGGTGGACAGTCCCCTTGGAGTTTAG(+CAGTGT)GATCCTGTGTCCCGTTCCCCACTTCGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605200946_n7yz GGTGCTGTTGGCACCACCACCCGAAGACATGG(+CAGTGT)GTACCTGTACCAGACTTCATAAGCAGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605200945_jaw7 TGGAGATGAGCTGCTTATGAAGTCTGGTACAG(+CAGTGT)GTACCCATGTCTTCGGGTGGTGGTGCCAACA NGG 10 8 17 8 24 0 100 5 10
    240605200943_1z0p CACCAGGCTAGGCTCATGGCCTTGAACTCAGG(+CAGTGT)GATCCACCTGCCTCTGCCTCCCTGTGATGCT NGG 10 8 17 8 24 0 100 5 10