Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605201029_6mpo AAACACTTATGTGCATTTCATTTTCATCCATG(+CAGTGT)GTACCTATTCTTCTAAACATGACATCAATTC NGG 10 8 17 8 24 0 100 5 10
    240605201027_t67d GCAAACAGCAAGAGGCTTTATTGAGAACACGG(+CAGTGT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605201024_wj5l GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+CAGTGT)GTACCCGTGTTCTCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605201022_uvp5 CACACGTGGCCTTTTACCGTGATGCTCCTGTG(+CAGTGT)GTACCTGCAGCTTCCGAGGGGAACTTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605201021_138a GACCCCACAGAAGTTCCCCTCGGAAGCTGCAG(+CAGTGT)GTACCACAGGAGCATCACGGTAAAAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605201019_ik5x GGGGAATTGCTAGGAGCAACTATGGCTGCCAG(+CAGTGT)GTACCCACCCCTCATCCTGGGCTCCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605201017_wuxm TCCTGGGCCAGGGAGCCCAGGATGAGGGGTGG(+CAGTGT)GTACCTGGCAGCCATAGTTGCTCCTAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605201016_nkc3 GGGCCACGGCATTACAAGAGGGGCTGAAGTGG(+CAGTGT)GTACCAGGCTTTTCCTCATTTTTGGTACAAG NGG 10 8 17 8 24 0 100 5 10
    240605201014_shkp GATCACTTGTACCAAAAATGAGGAAAAGCCTG(+CAGTGT)GTACCCACTTCAGCCCCTCTTGTAATGCCGT NGG 10 8 17 8 24 0 100 5 10
    240605201012_ad7j GGAAGGCTCCTGGCTCTAGGTCTGTGCCTCTG(+CAGTGT)GATCCACATTGGTCTTGGTCCATGTGGCACT NGG 10 8 17 8 24 0 100 5 10