Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200923_kt99 GCAGAGCAGCCAGGCTGGTACATGTGATGGAG(+CAGTGT)GATCCCAGGCTGGGCTGCCTCTAAAGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605200922_uegk ACATACACACAATCAAAGCTAGCACAAAAACG(+CAGTGT)GATCCCAAGTCCAAACTGATAAGAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240605200920_4scj GCACCCATCGTTTCTTATCAGTTTGGACTTGG(+CAGTGT)GATCCGTTTTTGTGCTAGCTTTGATTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605200919_412v TAGCACAACCATGCTAAGCAGGGGTTCATGAG(+CAGTGT)GTACCGGGGTCATTGAAGAGACCCCAGAATC NGG 10 8 17 8 24 0 100 5 10
    240605200917_t8u1 GTTGTGATTCTGGGGTCTCTTCAATGACCCCG(+CAGTGT)GTACCTCATGAACCCCTGCTTAGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605200913_p66q TAGTTTCAATGGTTTCTGAAGCATAGGCAGGG(+CAGTGT)GTACCCTGCTACTGAAACAGCAAGTCACTCT NGG 10 8 17 8 24 0 100 5 10
    240605200911_w9y5 CACTGAGAGTGACTTGCTGTTTCAGTAGCAGG(+CAGTGT)GTACCCCTGCCTATGCTTCAGAAACCATTGA NGG 10 8 17 8 24 0 100 5 10
    240605200908_z0se GGAAGAGGAGGGGCTTGTGCAGGAGCCCCCAG(+CAGTGT)GATCCAGTTAGTGCTGCATTCTTTTAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605200907_izc0 CTTGCCTGCCTAAAAGAATGCAGCACTAACTG(+CAGTGT)GATCCTGGGGGCTCCTGCACAAGCCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605200904_yoh5 AAGAAAATGACATGGCCTGGAGGAGATTCGGG(+CAGTGT)GTACCTTTCCTCAGTGAGCTTTGATGTGGTC NGG 10 8 17 8 24 0 100 5 10