Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200723_etng TCTAGGGGCAAGCCAGCTCAAGCAGCCTGAAG(+CAGTGT)GATCCCGGATTGGTGGCTGTTGGAATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605200721_a1cw TCATTTCTTCATTCCAACAGCCACCAATCCGG(+CAGTGT)GATCCTTCAGGCTGCTTGAGCTGGCTTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605200720_3ybf GCATGTATCAATACACATTACATTCATGCTTG(+CAGTGT)GTACCTGTGGAGGTCAGAAGGGGGCATCCGA NGG 10 8 17 8 24 0 100 5 10
    240605200718_0rqm GGGGATCGGATGCCCCCTTCTGACCTCCACAG(+CAGTGT)GTACCAAGCATGAATGTAATGTGTATTGATA NGG 10 8 17 8 24 0 100 5 10
    240605200716_lqp6 TCAATGGTGATTAGAGAAGCAAGCATTATATG(+CAGTGT)GATCCCAATCAGATAGAGAAATGCAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605200714_w2cp TTTCTTTTCTTTGCATTTCTCTATCTGATTGG(+CAGTGT)GATCCATATAATGCTTGCTTCTCTAATCACC NGG 10 8 17 8 24 0 100 5 10
    240605200712_8dk8 CACTCGCTGGTCCCAACCTCCTCATATGTGTG(+CAGTGT)GATCCTCAAGGAAGGCCATGATGATCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605200711_qxyd CCCAAGCCCTGATCATCATGGCCTTCCTTGAG(+CAGTGT)GATCCACACATATGAGGAGGTTGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605200709_c8x5 TGCAATTACTGCAATCCCTCTGAAAGTGCTTG(+CAGTGT)GATCCGCCGGCAAATATTAAAAGGAGATTTC NGG 10 8 17 8 24 0 100 5 10
    240605200707_q6rh TTGTGGAAATCTCCTTTTAATATTTGCCGGCG(+CAGTGT)GATCCAAGCACTTTCAGAGGGATTGCAGTAA NGG 10 8 17 8 24 0 100 5 10