Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200651_u4ee TATCAGCCAGTGTGGCCCTCTATTTCCTTGTG(+CAGTGT)GTACCGAGTGTCCCTGAGGCTGGGGTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605200650_0v4o GAAAAAGACCACCCCAGCCTCAGGGACACTCG(+CAGTGT)GTACCACAAGGAAATAGAGGGCCACACTGGC NGG 10 8 17 8 24 0 100 5 10
    240605200647_wn22 AGCATGCAGACATCCCTCTGTGGACTTCAGGG(+CAGTGT)GATCCACCTCTAAAGAGAGTCACTAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605200646_609j CTGTTGGTCACTAGTGACTCTCTTTAGAGGTG(+CAGTGT)GATCCCCTGAAGTCCACAGAGGGATGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605200644_q0s9 GGGTTCCCACGTGTCTGGAAGCTCGAGTTCCG(+CAGTGT)GATCCATACTGACACCCAATAAGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605200643_iz84 TACTGCTACACAGCTTATTGGGTGTCAGTATG(+CAGTGT)GATCCGGAACTCGAGCTTCCAGACACGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605200640_l85y AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+CAGTGT)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605200639_e2ap TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+CAGTGT)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    240605200638_w97l GCAGAACCTAGGGCTTTGACCATGGCTTAGTG(+CAGTGT)GTACCATGCGAAGACACTTAGTGTGACAAGT NGG 10 8 17 8 24 0 100 5 10
    240605200637_jsb8 CTCAGACTTGTCACACTAAGTGTCTTCGCATG(+CAGTGT)GTACCACTAAGCCATGGTCAAAGCCCTAGGT NGG 10 8 17 8 24 0 100 5 10